Aussie star Tomljanovic overcame her Latvian rival 4-6 6-4 6-2 but was furious in the third and final set when Ostapenko asked for the physio at 0-30 after losing seven games in a row.
Speaking to the umpire, Tomljanovic fumed: “Do you know that she is lying? We all know. Do you think that everything was fine with her for an hour and a half, but now suddenly there was an acute injury?”
Despite the pair seeing out the match, there was more bad blood when they met at the net.
“If you think I’m faking, you can talk to a physiotherapist,” sniped the 24-year-old Latvian.
“I hope you’re feeling better,” responded Tomljanovic sarcastically, before former French Open champion Ostapenko fired back: “Your behavior is terrible. You have no respect.”
The win for Croatian-born Tomljanovic, 28, the world number 75, set up a meeting with British teenage prodigy Emma Raducanu in the round of 16.
Even with that to look forward to, Tomljanovic was not done sticking the knife into Ostapenko.
“As far as she goes, look, I have been in situations where players use that medical timeout to get their opponent off, usually when they are losing badly and when it’s, like, the match was getting closer to the end,” she said.
“She can say she was injured. I don’t think she was. There was nothing wrong with her the whole match, but then why on 4-0 she calls it?
“So I think that that’s a clear reason that she just wanted to get me off my game. Usually, if you are dealing with an injury – at least, this is how I go about it – you maybe say, ‘hey, can you call the physio on the next changeover?’