The researchers grew a two-headed fish, controlling the expression of the required gene.
Japanese scientists from Hokkaido University have managed to grow two-headed fish, using the method accurate and fast control of gene expression, thereby proving its applicability. The main feature of this technique is that the effect occurs in RNA, not DNA.
Control of gene expression available technologies is limited by the speed of response and duration of effect. However, in recent years, methods of exposure with the light, but he turned out to be unsuitable for embryonic development due to the delay of several hours between exposure and caused by the beginning or termination of protein synthesis. Furthermore, existing technologies require genetic modification.
The Japanese geneticist Shinzi Ogasawara has proposed to intervene in the process of translating mRNA into protein, instead of the conventional effects on the transcription of DNA into mRNA. This eliminated the need to make genetic modifications — do mRNA was altered in such a way that they are associated with a specific signal molecule upon irradiation with ultraviolet light could not reach, being lit up blue.
As proof of this theory, scientists conducted an experiment by growing two-headed fish, controlling the expression of the required gene. In addition, it was shown that protein production starts after just a few minutes after you turn the UV light on and off a few tens of minutes after irradiation with blue light.
“Our method may be particularly useful for the control of embryonic development, and to identify the importance of beginning and duration of gene expression. Applying this technology to higher organisms such as mouse, we hope to clarify the role of each gene in the development of animals and various diseases,” said Ogasawara.
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